Giuseppe Querques



Authors: Giuseppe Querques (1), Alessandro Rabiolo (1); Francesco Gelormini (1); Alessandro Marchese (1); Maria Vittoria Cicinelli (1); Giacinto Triolo (1); Riccardo Sacconi (1,2); Lea Querques (1); Francesco Bandello (1)

Authors affiliation:

(1)  Department of Ophthalmology, University Vita-Salute, IRCCS San Raffaele, Milan, Italy

(2)  Eye Clinic, Department of Neurological and Movement Sciences, University of Verona, Verona, Italy.


Corresponding author: Giuseppe Querques, Department of Ophthalmology, IRCCS Ospedale San Raffaele, University Vita-Salute San Raffaele, Via Olgettina 60, Milan, 20132, Italy

Tel: +390226432648

Fax: +390226433643



The authors have no proprietary interest in the materials used in this study.

Short title: Macular perfusion parameters and scan size

Author contributions: AR and GQ designed the experiment; AR, FG, AM, MVC, LQ and RS conducted the experiment and analyzed/interpreted data; AR wrote the article; FB provided materials; AM, GT, FB and GQ proofed/revised article.


Funding/Support: NONE


Conflict of Interest: Alessandro Rabiolo, Alessandro Marchese, Francesco Gelormini, Maria Vittoria Cicinelli, Giacinto Triolo and Lea Querques have no disclosures. Giuseppe Querques consultant for: Alimera Sciences (Alpharetta, Georgia, USA), Allergan Inc (Irvine, California, USA), Bayer Schering-Pharma (Berlin, Germany), Heidelberg (Germany), Novartis (Basel, Switzerland), Sandoz (Berlin, Germany), Zeiss (Dublin, USA).  Francesco Bandello has the following disclosures: ALLERGAN (S), ALIMERA (S), BAYER (S), FARMILA-THEA (S), SCHERING PHARMA (S), SANOFI-AVENTIS (S), NOVAGALI (S), PHARMA (S), HOFFMANN-LA ROCHE (S), GENENTECH (S), NOVARTIS (S).


Keywords: optical coherence tomography angiography; quantitative optical coherence tomography angiography; foveal avascular zone; vessel density; superficial capillary plexus; deep capillary plexus; ImageJ.



Purpose. To investigate the macular quantitative parameters interchangeability of three different optical coherence tomography angiography (OCT-A) angiocubes  on healthy subjects and patients affected by diabetic retinopathy (DR).

Methods. Retrospective study involving 20 eyes of healthy subjects and 20 eyes with DR. All eyes underwent swept-source OCT-A with 3x3 mm, 6x6 mm and 12x12 mm angiocubes centered on the fovea. Foveal avascular zone (FAZ) area and vessel density on 3x3 mm, 6x6 mm and 12x12 mm macular scans were calculated by 3 independent operators at all retina, superficial, deep and choriocapillary vascular layers, and interchangeability and interrater reliabilities were estimated using intraclass correlation coefficient (ICC).

Results. Interscan reproducibility of FAZ area was very strong (ICC > 0.85) at every plexus. Vessel density values significantly varied across different scan sizes (ICC < 0.51). Intrascan interrater reliability was high for all retina and superficial FAZ areas, while it was satisfactory at deep capillary plexus only for 3x3 mm scan.

Conclusions. FAZ area at all plexuses is a robust parameter even if calculated on angiocubes with different size, even though interrater reliability is higher when measured in smaller scans. Conversely, vessel density depends on the size of angiocube, although their interrater reliability is extremely high.